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Figure 3
Mineralization assay on stem cell differentiation. (a) Alizarin red S red staining of mouse MSCs (C3H10T1/2) showing calcium mineralization during week 1. Photomicrographs clearly illustrating the formation of mineralized nodules in differentiated osteoblasts. Scale bar: 25 µm. (b) Bar diagram displaying absorbance values (405 nm) for Z-VA on Alizarin red S staining during 7 days compared to untreated control/VA. *Statistically significant compared to control (
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