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Figure 5
Effect of the anti-osteoporotic agent Z-VA on scale mineralization. (a) Regenerated scales observed from the Alizarin red S red staining assay exhibit red nodules, indicating calcium deposition facilitated by osteoblast migration from the focal region toward the periphery. (b) Photomicrograph from the von Kossa staining assay reveals calcified brown-colored scales with calcium and phosphate mineralization. Non-mineralized regions resulting from osteoclastic matrix degradation are highlighted by white-colored resorption pits. Bar diagram depicting the absorbance of (c) Alizarin red S red and (d) von Kossa stained scales, illustrating calcification during week 4. *indicates a significant increase compared to control/Dexa, while #indicates a significant decrease compared to control (