Figure 1

Figure 1

Effect of DMF and its derivatives on the viability and nitric oxide (NO) output in the BV‐2 microglia cell model. (A) Cell viability examined by the CCK‐8 assay, showing identical cytotoxic profiles of DMF and IDMF, and lack of cytotoxicity up to 100 μm for DRF (*P < 0.05 Diroximel vs. IDMF and DMF). (B–D) IDMF (B) inhibits the production of nitric oxide (NO) significantly better than DRF (D) at 2.5 μm and higher doses (P < 0.05). Furthermore, IDMF inhibits the production of NO significantly better than DMF (C) at 5 μm and up. ##P < 0.05 LPS‐stimulated vs. non‐stimulated controls; **P < 0.05 vs. LPS‐stimulated control. Error bars indicate standard deviations. N value: 3. P values were calculated using two‐tailed t‐test.