|
Fig. 5 Effect of F0 generation female or male fish exposure to difenoconazole on the development of the hybrid F1 generation embryos/larvae. (A) Number of spontaneous movement in 20 s of embryos at 24 hpf; (B) heartbeat of 48 hpf embryos within 20 s; (C) malformation rate of 48 hpf embryos; (D) hatching rate of F1 generation at 72 hpf; (E) body length of F1 generation at 120 hpf; (F) mortality of F1 generation at 24 hpf and 120 hpf. Female represents F1 generation embryos/larvae produced by the pairing of difenoconazole-exposed F0 generation females and nondifenoconazole-exposed males; male represents the F1 generation embryos/larvae produced by the pairing of difenoconazole-exposed F0 males and nondifenoconazole-exposed females. (*p < 0.05; **p < 0.01).