Fig 3

Fig 3 Zebrafish s100s do not exhibit nutritional immunity activity like human calprotectin.

(A) Dose dependence of human calprotectin challenge on human and zebrafish commensal bacteria. Each column specifies which bacteria was used for the set of nutritional immunity assays: human-derived Gram-positive Stapholococcus epidermidis and two zebrafish-derived Gram-negative bacteria, Aeromonasstrain: ZOR001 andVibrio strain: ZWU0020. Bacterial growth was measured by OD₆₀₀ over 13 hours with challenge by increasing doses of human calprotectin noted in the legend on the right. Concentration increases from dark blue to dark green and black arrows indicate how growth is affected as calprotectin increases. Error bars indicate standard error of three biological replicates. (B) Zebrafish s100 dose effects on human and zebrafish commensal bacterial growth compared to human calprotectin. Each datapoint shows the change in the area under the curve from the absence of s100 protein to the indicated s100 concentration, measured from growth curves like those shown in panel A (S4 Fig). The dotted line at zero represents no effect on bacterial growth.