|
Fig. 2 Lateral mesendoderm cells migrating on the ectoderm display most-pronounced animal migration (A) Correlation between the time lateral mesendoderm (LME) and endoderm (Endo) cells migrate on the ectoderm (x axis) and their displacement along the animal-vegetal (AnVeg) axis, expressed as final y-displacement from the origin (y axis). Green dots, LME; orange dots, Endo cells. Statistical test, Spearman r. Number of pairs, 259 (163 LME cells from 3 embryos; 96 Endo cells from 2 embryos). See also Figure S2. (B) Explanation of the subgrouping used for the analyses in (C)–(F) and in Figure S2. Cells using ectoderm as substrate for either more than 70% or less than 30% of their migration time (see also graphs in Figures S2F and S2G) were defined in the analysis as cells migrating mostly on the ectoderm (Ecto) or mostly on the yolk cell (YC) membrane (Yolk), respectively. (C) Total displacement of LME cells using mostly the ectoderm (Ecto, dark green) or the YC membrane (Yolk, light green) as substrate for their migration. Number of cells: Ecto, 99; yolk, 42. Number of embryos, 3. Statistical test, Mann-Whitney test: ∗∗∗∗p < 0.0001. (D) Migration persistence of LME using mostly the ectoderm (Ecto, dark green) or the YC membrane (Yolk, light green) as substrate for their migration. Number of cells: Ecto, 99; yolk, 42. Number of embryos, 3. Statistical test, Mann-Whitney test: ∗∗∗∗p < 0.0001. (E) Migration directionality of LME cells using mostly the ectoderm (Ecto, dark green) or the YC membrane (Yolk, light green). An, animal; Veg, vegetal; D, dorsal; V, ventral. Number of cells: Ecto, 99; yolk, 42. Number of embryos, 3. (F) Displacement (y displacement) of LME cells along the AnVeg axis over time. Solid line represents the mean; gray ribbon displays confidence interval. LME mostly using the ectoderm (Ecto) or the YC membrane (Yolk) as substrate for their migration are shown in dark green and light green, respectively. Number of cells: Ecto, 99; yolk, 42. Number of embryos, 3. Statistical test on the final y displacement, Mann-Whitney test: ∗∗∗∗p < 0.0001.