IMAGE

Fig. 5

ID: ZDB-IMAGE-250428-144
Publication: Yong et al., 2025 - Cryo-EM structure of the BLOC-3 complex provides insights into the pathogenesis of Hermansky-Pudlak syndrome
All Figures: Figures for Yong et al., 2025

Image

Figure Caption

Fig. 5 The interaction between BLOC-3 and RAB9A.a Low-resolution cryoEM envelope of BLOC-3-Rab9A Q66L with docked AlphaFold3 model. b Close-up view of the HPS4-RAB9A binding interface, with critical residues for RAB9A binding shown in sticks. Residues that are mutated in (d) are indicated with a yellow box. c Flag-tagged HPS4 WT and mutants were expressed and purified using HEK293T cells, then incubated with purified His-tagged mouse Rab9A Q66L mutant and subjected to Flag pull-down. HPS4 and Rab9A were detected by antibodies against Flag and His, respectively. d Quantification of the results presented in (c). Statistical differences were determined by One-way ANOVA. Error bars represent the standard deviation of three independent biological repeats. n = 3.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.