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Fig. 6 Condensin II subunits are required for HSPC maintenance. (A–C) WISH using the cmyb antisense probe at 72 hpf in WT embryos injected with morpholinos (MOs) targeting smc4 (A), condensin I subunits (ncaph1, ncapg1, and ncapd2) (B), condensin II subunits (ncaph2, ncapg2, and ncapd3) (C), and their corresponding uninjected controls. Black square brackets indicate cmyb-positive HSPCs in the CHT. The numbers shown at the bottom of the images represent the count of representative outcomes observed relative to the total number of embryos obtained. WISH data from a single experiment. (D) Confocal microscopy images of EdU-stained morphants of ncapg1 and ncapg2 and their uninjected controls at 72 hpf. White-dotted box indicates CHT. Bottom panel: quantification of EdU+ cells in the CHT (uninjected, n = 10; ncapg1-MO, n = 10; ncapg2-MO, n = 10; n.s., no significance at p > 0.05; **p < 0.01). Data from a single experiment. (E) Confocal microscopy images of H3P-stained morphants targeting ncapg1 and ncapg2 and their uninjected controls at 72 hpf. Bottom panel: quantification of H3P+ cells in the CHT (uninjected, n = 10; ncapg1-MO, n = 10; ncapg2-MO, n = 10; n.s., no significance at p > 0.05; ***p < 0.001). All quantification data were quantified and expressed as the mean ± SEM. Statistical analysis was performed using a t-test to calculate p-values. Data from a single experiment. Scale bar = 200 μm.