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Fig. 8 DMH1-treated larvae have significantly more ampullary organs at stage 45 than older wild-type larvae. (A,B) Scatter plots showing median and interquartile range for the number of ampullary organs on one side of the head in stage 45 sterlet larvae that had been treated for 20 hr from stage 36 with DMH1 (n=17) vs 2.0/2.8 cm wild-type larvae (~50/65 dpf; n=10). Raw counts are provided in Supplementary file 3. Two-tailed Mann-Whitney tests were used for statistical analysis. DMH1-treated larvae have significantly more ampullary organs overall at stage 45 than wild-type older larvae (p<0.0001; panel A). In panel B, scatter plots are grouped with differently coloured titles according to lateral line placode (LLp) origin, following Gibbs and Northcutt, 2004: blue, anterodorsal LLp (supraorbital and infraorbital fields); orange, anteroventral LLp (preopercular fields); green, otic LLp (dorsal otic field); pink, supratemporal LLp (supratemporal field). DMH1-treated larvae have significantly more ampullary organs at stage 45 than older wild-type larvae in all fields except the ventral supraorbital and posterior preopercular fields. Symbols on plots represent p values: ns, not significant, (p>0.05; *, p≤0.05; **, p≤0.01; ***, p≤0.001; ****, p≤0.0001). Dorsal supraorbital: p<0.0001. Ventral supraorbital: not significant (p=0.5109). Dorsal infraorbital: p=0.0123. Ventral infraorbital: p=0.0002. Anterior preopercular: p=0083. Posterior preopercular: not significant (p=0.1789). Dorsal otic: p<0.0001. Supratemporal: p<0.0001. (C) Schematic of a stage 45 sterlet larval head. Ampullary organ fields are represented by coloured patches flanking the neuromast lines, which are represented as dotted lines. The different field colours indicate their lateral line placode origin (consistent with scatter plot titles in B). Abbreviations for ampullary organ fields: app, anterior preopercular; di, dorsal infraorbital; dot, dorsal otic; ds, dorsal supraorbital; ppp, posterior preopercular; st, supratemporal; vi, ventral infraorbital; vs, ventral supraorbital. Abbreviations for neuromast lines: io, infraorbital; m, middle; ol, otic; pop, preopercular; so, supraorbital; st, supratemporal. Other abbreviations: an, anterior naris; b, barbel; e, eye; m, mouth; op, operculum; ot, otic vesicle; pn, posterior naris; s, spiracle (first gill cleft); WT, wild type.