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Fig. 5 Bmp5 crispants have significantly fewer ampullary organs than control Tyr crispants. (A) Scatter plot showing median and interquartile range for the total number of ampullary organs on one side of the head at stage 45 in Bmp5 sterlet crispants (counted after in situ hybridisation [ISH] for Cacna1d or Kcnab3; n=36) versus control Tyr crispants (counted after ISH for Cacna1d or Kcnab3; n=32). Bmp5 crispants have significantly fewer ampullary organs overall than control Tyr crispants (p<0.0001; two-tailed Mann-Whitney test). Supplementary file 2 provides the sgRNA combination, injection batch and raw counts for each crispant. All the Bmp5 crispants and 20 of the Tyr crispants used for statistical analysis were from the same batch. (B) Scatter plots showing median and interquartile range for the number of ampullary organs in each individual ampullary organ field on one side of the head at stage 45 in Bmp5 crispants (n=36) versus control Tyr crispants (n=32). The raw counts are provided in Supplementary file 2. For the location of each field, see panel C (Cacna1d expression) and panel D (schematic). Scatter plots are grouped with differently coloured titles according to lateral line placode (LLp) origin, following Gibbs and Northcutt, 2004: blue, anterodorsal LLp (supraorbital and infraorbital fields); orange, anteroventral LLp (preopercular fields); green, otic LLp (dorsal otic field); pink, supratemporal LLp (supratemporal field). All fields except the dorsal supraorbital field have significantly fewer ampullary organs in Bmp5 crispants versus control Tyr crispants (two-tailed Mann-Whitney tests). Symbols on plots represent p values: ns, not significant, p>0.05; *, p?0.05; ***, p?0.001; ****, p?0.0001. Dorsal supraorbital: not significant, p=0.1207. Ventral supraorbital: p=0.0008. Dorsal infraorbital: p<0.0001. Ventral infraorbital: p<0.0001. Anterior preopercular: p=0.0466. Posterior preopercular: p<0.0001. Dorsal otic: p=0.0008. Supratemporal: p<0.0001. (C) Stage 45 sterlet head after ISH for the hair cell and electroreceptor marker Cacna1d (also expressed in taste buds on the barbels). Labels are white for ampullary organ fields; black for neuromast lines; yellow for anatomical landmarks. (D) Schematic of a stage 45 sterlet larval head. Ampullary organ fields are represented by coloured patches flanking the neuromast lines, which are represented as dotted lines. The different field colours indicate their lateral line placode origin (consistent with scatter plot titles in B). Abbreviations for ampullary organ fields: app, anterior preopercular; di, dorsal infraorbital; dot, dorsal otic; ds, dorsal supraorbital; ppp, posterior preopercular; st, supratemporal; vi, ventral infraorbital; vs, ventral supraorbital. Abbreviations for neuromast lines: io, infraorbital; m, middle; ol, otic; pop, preopercular; so, supraorbital; st, supratemporal. Abbreviations for anatomical landmarks: an, anterior naris; b, barbel; e, eye; m, mouth; op, operculum; ot, otic vesicle; pn, posterior naris; s, spiracle (first gill cleft).