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Fig. 3 - Supplemental 2 Sterlet Sostdc1 is expressed in neuromasts but only transiently in ampullary organs. In situ hybridisation in sterlet for Sostdc1, encoding a secreted dual Wnt/Bmp antagonist. Black arrowheads indicate examples of developing neuromasts; white arrowheads indicate examples of developing ampullary organs. For trunk images, boxes on low-power insets delineate the regions shown. Non-lateral line expression is seen in developing gill filaments. (A–D) Sostdc1 expression is seen in neuromasts on the head from stage 36 (A,B), persisting at stage 38 (C,D). (E–G) At stage 40, expression is maintained in neuromasts on the head (E,F) and is also visible in the three migrating lateral line primordia on the trunk and in trunk neuromasts (G). (H–J) At stage 42, Sostdc1 expression persists in cranial neuromasts and is now also seen in ampullary organs (H,I). On the trunk, expression persists in the migrating primordia and neuromasts (J). (K–M) By stage 45, only neuromast expression is observed on the head (K,L). On the trunk, expression continues in the migrating primordia and neuromasts (M). Abbreviations: e, eye; prim, migrating lateral line primordium (primI, primary; primII, secondary; primD, dorsal); S, stage. Scale bar: 250 μm.