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Fig. 2 - Supplemental 1 Photoactivation of one or more cells by caged cyclofen (cCYC) uncaging. At 1 day post-fertilization (dpf), an area of diameter ~80 ?m in the vicinity of the otic vesicle (white arrow) was illuminated for 7 min at 405 nm, uncaging cCYC and resulting in one or a few cells in the illuminated region to express the oncogene kRASG12V and its blue fluorescent marker (H2B-mTFP). In these conditions single-cell activation represents about 50% of the cases (see Figure 2).
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