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Fig. 2 Embedding and antigen retrieval methods affect WGA staining in rat heart. Hearts from control and TAC subjected rats were cut frozen or paraffin embedded and subjected to citrate, Tris or proteinase K-based antigen retrieval. Sections were then stained with WGA lectin in 1:50, 1:100 or 1:200 dilution. Representative images are shown. Scale bars = 100 ?m. FFPE, formalin fixed paraffin embedded; TAC, transverse aortic constriction.
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