ZFIN Image: Huybrechts et al., 2025, Fig. 6

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Figure Caption

Fig. 6 Osteoclast histomorphometry in scales from wild-type and sqstm1tmΔUBA zebrafish at 6 mpf. a Representative images of Von Kossa (top row) and TRAP staining (bottom row) of elasmoid scales from 2 different sqstm1+/+, sqstm1tmΔUBA/+ and sqstm1tmΔUBA/tmΔUBA at 6 mpf. Non-mineralization, i.e., bone resorption and TRAP-positivity are shown by arrowheads and arrows, respectively. Black spots indicate staining artifacts. b Quantification of circularity, c relative resorbed area over total scale area, and d relative TRAP-stained area over total scale area. Each data point represents an individual scale. n = 4 zebrafish/genotype and n = 9 (on average) scales/zebrafish/staining. Statistical analysis was performed using Kruskal–Wallis Tests/Mann–Whitney U Tests. WT wild type (sqstm1+/+), HET heterozygous mutant (sqstm1tmΔUBA/+), HOM homozygous mutant (sqstm1.tmΔUBA/tmΔUBA)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Calcif. Tissue Int.