ZFIN Image: Huybrechts et al., 2025, Fig. 5

Image

Figure Caption

Fig. 5 Osteocyte evaluation in the vertebral column of wild-type and sqstm1 mutant zebrafish. a Representative images after Masson’s Trichrome staining of vertebral column sections of a wild-type and homozygous mutant zebrafish at 6 mpf (ventral side below, scale bar = 100 µm). b Magnification of the black square in (a). Examples of osteocyte lacunae classified as “non-enlarged” and as “enlarged” are indicated by red and black arrow heads, respectively. c Quantification of the total osteocytes per unit area of bone matrix. Each data point represents the average of 2 precaudal or caudal fields per zebrafish. d Fraction of the number of enlarged osteocyte lacunae over the total number of osteocytes. Each data point represents the average of 2 precaudal or caudal fields per zebrafish. n = 4 zebrafish/genotype/age. Statistical analysis was performed using Kruskal–Wallis Tests/Mann–Whitney U Tests. WT wild type (sqstm1+/+), HET heterozygous mutant (sqstm1tmΔUBA/+), HOM homozygous mutant (sqstm1.tmΔUBA/tmΔUBA)

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Calcif. Tissue Int.