ZFIN Image: Dong et al., 2025, Fig. 3

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Figure Caption

Fig. 3

Impact of Bifidobacterium longum subsp. longum BL21 on sperm parameters in zebrafish experiencing cyclophosphamide-induced reproductive dysfunction. (A) Sperm count, (B) Viability, (C,D) DNA fragmentation. The groups are as follows: CTL (normal control), CS (cyclophosphamide-only), BL21 (BL21 treatment), and CC (positive control with clomiphene citrate). ***p < 0.001. FITC-H denotes fluorescence intensity of stained DNA, reflecting DNA fragmentation levels, and SSC-H represents side scatter height, assessing cellular complexity.

Acknowledgments

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