Fig. 4

Fig. 4 Combination treatment with SL-176 and GSK-J4 induces vast transcriptional changes in neuroblastoma cells.

A volcano plots showing differentially expressed genes in IMR-32 cells treated for 72 hours with 3 µM SL-176, 0.3 µM GSK-J4, or the combination, as compared to vehicle-treated cells. B Venn diagrams showing up- and downregulated genes in IMR-32 cells (Log₂fold change ≥ 1 or ≤ 1, respectively). C list of top enriched or downregulated gene sets for IMR-32. D volcano plots showing differentially expressed genes in SK-N-BE(2) cells treated for 72 hours with 5 µM SL-176, 0.5 µM GSK-J4, or the combination, as compared to vehicle-treated cells. E Venn diagrams showing up- and downregulated genes in SK-N-BE(2) cells (Log₂fold change ≥ 1 or ≤ 1, respectively). F list of top enriched or downregulated gene sets for SK-N-BE(2). G Venn diagrams showing common upregulated (Log₂fold change ≥ 1) or downregulated genes (Log₂fold change ≤ 1) between IMR-32 and SK-N-BE(2) cells. In A and D, -Log10 P_adj values > 100 were set at 100.