Fig. 3

Fig. 3 Combined treatment with SL-176 and GSK-J4 induces cell cycle arrest and apoptosis.

A immunoblots of neuroblastoma cells treated for 48 hours with vehicle, SL-176, GSK-J4 or the combination. B corresponding densitometry of bands seen in (A), normalized to vinculin, or, in the case of phosphorylated p38, to total p38. C immunoblots of neuroblastoma cells treated for 72 hours with vehicle, SL-176, GSK-J4 or the combination. D corresponding densitometry of bands seen in (C), normalized to vinculin, or, in the case of phosphorylated p38, to total p38. Protein expression was investigated with immunoblot and the experiments were repeated with similar results. Please refer to the supplementary immunoblot material for complete immunoblots and Ponceau stainings. E relative expression of TP53, BBC3 and CDKN1A in IMR-32 and SK-N-BE(2) neuroblastoma cells treated with either vehicle, SL-176 (3–5 µM), GSK-J4 (0.3–0.5 µM) or the combination for 6–72 hours, analyzed with qPCR. The mean of technical triplicates is presented.