Figure 2

Figure 2

Impaired bone mineralization and abnormal vitamin B₆ metabolism in alpl knockout zebrafish. (a) Decreased alizarin red staining of mineralized structures (notochord, indicated by arrows) in alpl^-/- compared to WT and alpl^+/- embryos at 5 dpf suggests a negative effect of alpl deficiency on bone mineralization. (b) Accumulation of d3-PLP and (c) decreased production of d3-pyridoxal (d3-PL) in alpl^-/- compared to WT and alpl^+/- embryos incubated with 100 µM d3-PLP in embryo water (E3) for 3 h at 28 °C. Data are means from n = 3 pools (3 embryos per pool) per time point and genotype ± SD. (d) Steady-state PLP, (e) pyridoxal (PL), (f) 4-pyridoxic acid (4-PA), (g) pyridoxamine 5′-phospate (PMP), (h) pyridoxamine (PM), (i) pyridoxine 5′-phosphate (PNP), and (j) pyridoxine (PN) concentrations in whole embryo extracts. Data are means from n = 8 pools (3 embryos per pool) per genotype ± SD. **** p < 0.0001, *** p < 0.001 ** p < 0.01, * p < 0.05, and ns—not significant (p > 0.05).