FIGURE 4

FIGURE 4

Response of regeneration‐response enhancers (RREs) and regeneration genes after cryoinjury. (a) Absence of msxc induction at 2 days post injury (dpi) in the Tg(msxc:egfp). n = 5. (b) Expression of EGFP in the Tg(fn1b:egfp) at 2 dpi. Tissue section showed that EGFP expression is observed in the epidermal keratinocytes as in the regenerating fin. n = 5. (c) Induction of junbb at 2 dpi as detected by in situ hybridization (ISH). The ISH signal is seen in the fin ray mesenchymal cells. n = 5. (d) Response of Tg(−1.8fn1b:egfp) to the cryoinjury at 2 dpi. Tissue sections revealed that mesenchymal cells that occupied fin ray expressed EGFP. n > 15. (e) Absence of response in the Tg(E2S‐miniP:egfp) after cryoinjury. n = 5. Scale bars, 100 μm (whole‐mount) and 50 μm (section). Dotted circles mark the cryoinjured regions.