FIGURE 1

FIGURE 1

Transgenic (Tg) line used in this study. (a) Response of Tg(−1.8fn1b:egfp) (1.8 k Tg) to fin amputation. The response was observed in mesenchymal cells of regenerating fin. Note that the response extends proximal to the amputation plane (Bracket region). (b) Response of Tg(E2S‐miniP:egfp) (E2S Tg) to fin amputation. The response was observed in the wound epidermis, strongly in the basal layer of the epidermis. (c) Response of Tg(fn1b:egfp) to fin amputation. EGFP is expressed in the entire wound epidermis including the superficial keratinocytes. (d) msxc expression during fin regeneration by whole‐mount in situ hybridization (ISH) (left panel) and by Tg(msxc:egfp). EGFP expression recapitulates the msxc expression, and is seen in the distal region of the blastema. EGFP fluorescence was observed from 1 day post amputation (dpa) to 8 dpa. Note that the msxc⁺ cells do not overlap with the zns5⁺ osteoblasts (section). Arrowheads denote the respective amputation planes. Scale bars, 100 μm (whole‐mount) and 50 μm (sections). n > 10, respectively.