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Fig. 4 The apoptosis of pronephric tubules and cloaca was significantly decreased upon gdf11 deletion. (A,B) TUNEL assay showing decreased apoptosis in cells of the developing pronephric duct and cloaca in gdf11 mutants. The position marked by the dotted line in the figure is the pronephros and the cloaca. Scale bar: 50 μm. (C,D) Cell proliferation, indicated by BrdU staining, remains unchanged in both WT and gdf11 mutants. The position marked by the dotted line in the figure is the pronephros and the cloaca. Scale bar: 50 μm.
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