ZFIN Image: van Bree et al., 2024, Fig. 6

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Figure Caption

Fig. 6 Transplantation in the blastula stage of zebrafish embryos can be used as an orthotopic medulloblastoma model for rapid in vivo testing of drug compounds. (A) Schematic overview of the treatment protocol. (B) Luciferase activity of transplanted tertiary tNES cells (C) and UW228-3 cells after 48 h of treatment with sonidegib or 4-HCP. Data normalized to the DMSO control and presented as mean ± SD with each point representing one zebrafish embryo of 2 individual experiments. *P < .05, **P < .01, 1-way ANOVA with Dunnett post-hoc test. (D) Representative images of zebrafish upon Sonidegib and 4-HCP treatment. Green indicates the vasculature system of zebrafish (fli1:EGFP) and red (Cy3) indicates the tumor cells transplanted.

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