Fig. 6

Fig. 6 GDP-L-fucose and GFP-tagged human FCSK rescue early development and fucosylated protein levels in fcsk−/− zebrafish A: Body length and statistical analysis of WT (injected with H2O), WT (injected with GDP-L-fucose), fcsk−/− (injected with H2O), and fcsk−/− (injected with GDP-L-fucose) zebrafish (5 dpf, n=24). Scale bar: 200 µm. B: Western and lectin blot analyses of WT (injected with H2O), WT (injected with GDP-L-fucose), fcsk−/− (injected with H2O), and fcsk−/− (injected with GDP-L-fucose) zebrafish (48 hpf). C: Body length and statistical analysis of WT (injected with pEGFP-C1), WT (injected with GFP-tagged human FCSK), fcsk−/− (injected with pEGFP-C1), and fcsk−/− (injected with GFP-tagged human FCSK) zebrafish (5 dpf, n=19). Scale bar: 200 µm. D: Western and lectin blot analyses of WT (injected with pEGFP-C1), WT (injected with GFP-tagged human FCSK), fcsk−/− (injected with pEGFP-C1), and fcsk−/− (injected with GFP-tagged human FCSK) zebrafish (72 hpf). Mean±SEM. ns: Not significant; *: P<0.05; ***: P<0.001.