Fig. 3

Fig. 3 Differential expression of piRNAs in sperm of males exposed to High and Low male-male competition environments.

A Principal component analysis showing variation across all samples used in the piRNA differential expression analysis. B Heatmap of normalised counts (log² CPM) from DESeq2 output of sRNA-seq data on piRNAs across all samples, grouped by treatment. C Heatmap of all significantly DE piRNAs clusters log² fold change (p = <0.05 are denoted by *) for pair-wise comparisons. Colour key shows piRNA expression level, and shows general trend of piRNA expression. PiRNA names are abbreviated (see Supplementary File S1 for full piRNA cluster loci names).