FIGURE 4

FIGURE 4

Neurotranscriptomic effects of lrrtm4l1 deletion. (a) Principal component analysis plot of the 200 most variable genes after differential expression analysis between lrrtm4l1^−/− (KO) and lrrtm4l1^+/+ (WT) zebrafish. (b) Heatmap of differentially expressed genes (DEGs; padj < 0.05 and LFC > |0.58|) between KO and WT zebrafish. Hierarchical clustering of samples and genes reveals large differences between KO and WT, but similar transcriptional patterns within the two lines. (c) Volcano plot displaying the DEGs between KO and WT. Selected genes have been highlighted. Golden dots indicate genes upregulated in KO more than log fold change 0.58, blue dots represent genes downregulated in HAZ more than LFC—0.58 and black dots represent genes not passing these thresholds as indicated by vertical dotted lines. The horizontal dotted line indicates the significance threshold (padj 0.05) (d) Functional annotation clustering using DAVID pathway analysis revealed a significantly enriched cluster (enrichment score ≥ 1.3) related to semaphoring‐plexin signaling. (e) g:Profiler functional enrichment analysis revealed enriched KEGG pathways related to fatty acids and amino acids. n = 4/group.