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Fig. 7 Neither the transient overexpression of tcap (a) nor nphp4 (b) reduced the PCE ratio of SH2E+/G59R fs incross larvae at 5 dpf. (c, d)When treated with PD184352 (an MAPK/ERK signaling pathway inhibitor) from 10 hpf, much more SH2E+/G59R fs incross larvae developed PCE at 5 dpf, while with BAY11-7082 (an NF-κB signaling pathway inhibitor), significant fewer larvae developed PCE (e, f). g, h The number of 5 dpf SH2E+/G59R fs incross larvae with PCE increased after AKT inhibitor VIII administration. All experiments were performed 3 times independently, *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the solvent control (the 0-concentration group). For (c, e, g), SH2E+/+ (from the same pair of parents as SH2E+/G59R fs) incross as the control. For (d, f, h), scale bars = 200 μm.