Fig. 4 a The spatiotemporal expression of functionable SH2E mRNA in SH2E+/+, SH2E+/G59R fs and SH2EG59R fs/G59R fs derived from the same SH2E+/G59R fs incross. b The relative expression of functionable SH2E mRNA in SH2E+/G59R fs incross larvae with/without PCE at 5 dpf (15 embryos pooled per sample, n = 3). c The survival of SH2E+/G59R fs incross larvae within 12 dpf. d The morphology of 5 dpf SH2ES82R fs/S82R fs, SH2EΔ12/Δ12 and SH2EΔ6/Δ6 larvae. e The SH2E mRNA level in SH2E+/S82R fs incross larvae with PCE at 5 dpf (15 embryos pooled per sample, n = 3). For (b, e), *P < 0.05 and ***P < 0.001 compared with the normal phenotype group.
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