Figure 1

Figure 1

Detection of phascogale PrP^C with anti-PrP monoclonal antibodies. (A) IlFigurelustration of the prion protein and anti-PrP antibody epitope binding sites. SP = signal peptide; OR = octapeptide repeat region; HR = hydrophobic region; Gly = N-glycosylation sites; S-S; disulphide bridges. Antibodies: SAF32, SAF72, ICSM18. (B) Western blot analysis of 10% (w/v) P. calura brain homogenates treated with (+) or with no proteinase K (PK) using anti-PrP antibodies SAF32 and SAF70. (C) Western blot analysis of 10% (w/v) P. calura, mouse (M. musculus), hamster (Cricetinae), and human (H. sapiens) brain homogenates using anti-PrP antibodies SAF32 and SAF70. (D) Western blot analysis of 10% (w/v) P. calura brain homogenates derived from adult male breeders (AMB), adult male non-breeders (AMNB), male juvenile (MJ), a female juvenile (FJ), and an adult female (AF) using anti-PrP antibodies SAF32 and SAF70. Representative of 3 independent experiments.