Figure 12

Figure 12

WISH results in WT and the asb5-deficiency group zebrafish embryos at 22 SS. (A–A′) The schematic diagram illustrates the expression of lefty1 in the LPM of the embryos. (B–B′′′) This diagram depicts spaw expression in proximity to the LPM. In the WT group, spaw is localized on the left side of the LPM, whereas the asb5-deficiency group displays three distinct expression patterns: left-side expression, bilateral expression, and absence of expression. (C–C′′′′) The schematic diagram shows lefty2 expression near the LPM in embryos. In the WT group, lefty2 is expressed on the left side of the LPM, while the asb5-deficiency group reveals four expression patterns: left-side expression, bilateral expression, reverse expression, and no expression. LPM: lateral plate mesoderm; Red triangular arrow: positive expression region; Scale bar = 500 µm (back up view).