WISH results for other genes related to L-R asymmetric development in zebrafish embryos at 48 hpf across various groups. Images (A–A″) illustrate the WISH expression of has2 in WT, asb5-deficiency group, and asb5-deficiency group + asb5a/asb5b-mRNA group (back view). Images (B–B″) show the WISH expression of foxa3 across different groups (back view). Images (C–C″) depict the WISH expression of notch1b in various groups (abdominal view). Images (D–D″) present the WISH expression of prrx1a in the same groups (abdominal view). 22 SS: 22 somite stage; green H: head; white B: back; red E: eyes; yellow HV: heart valves; Red scale bar = 500 µm; Green scale bar = 250 µm.
Acknowledgments
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