Figure 1

Figure 1

The impact of asb5a/asb5b deficiency on L-R asymmetry in cardiac development. (A–F) Representative images of the pericardial cavity in various groups at 48 hpf during embryonic development; scale bar = 200 µm (side view). (A′–F′) Representative images of heart positioning in different zebrafish groups at 48 hpf under the myl7:EGFP background; scale bar = 100 µm (belly up view); (A″–F″) Magnified images of the atria and ventricles in various zebrafish groups at 48 hpf, also under the myl7:EGFP background, scale bar = 50 µm (belly up view). The red triangular arrows indicate areas of enlargement in the cardiac pericardial cavity. BF: bright field; GF: green fluorescence; 10X: field of view under a 10× microscope; 20X: field of view under a 20× microscope; red E: eyes; red V: ventricle; yellow A: atrium; D-loop: dextral looping; N-loop: no looping; L-loop: levo-looping.