FIGURE 7

FIGURE 7

Quantification of phospho-histone H3 and 5-bromo-2′-deoxyuridine (BrdU) staining in control, heterozygous crim1^+/−, and crim1^−/− larvae that are homozygous for a 2 base pair deletion, c.339_340delCT p.Leu112Leufs*3, at 48 and 72 h post fertilization (hpf). (A) The quantification of the PH3 antibody showed no significant difference between control, heterozygous crim1^+/−, and crim1^−/− larvae. A total of 14 control larvae were scored, 11 crim1^+/− larvae were scored, and 8 crim1^−/− larvae were scored at 48 hpf. A total of 19 control larvae, 8 crim1^+/− larvae, and 17 crim1^−/− larvae were scored at 72 hpf. (B) The quantification of the BrdU antibody showed a significant reduction in BrdU staining in the homozygous crim1^−/− larvae compared to control and heterozygous crim1^+/− larvae at 48 hpf (P = 0.013) but not at 72 hpf. A total of eight control larvae were scored, three crim1^+/− larvae were scored, and seven crim1^−/− larvae were scored at 48 hpf. A total of five control larvae, five crim1^+/− larvae, and five crim1^−/− larvae were scored at 72 hpf.