FIGURE 3

FIGURE 3

Representative images of zebrafish eyes stained with H&E from control and crim1^−/− larvae that are homozygous for a 2 base pair deletion, c.339_340delCT p.Leu112Leufs*3, at 3–5 days post fertilization (dpf). The top row shows control larvae at 3 dpf (A), 4 dpf (B, C), and 5 dpf (D), showing normal lens and eye development. The bottom row shows homozygous crim1^−/− larvae at 3 dpf (E), 4 dpf (F, G), and 5 dpf (H), showing reduced lens and eye size in the crim1^−/− lenses at 3 and 4 dpf (E, F). At 3 dpf, 6–8 larvae were scored, and at 4 and 5 dpf, 10–12 larvae were scored. Scale bar = 50 µm.