Fig 12

Fig 12 Re-expression of Clrn1 in rod photoreceptors does not reduce sensitive to light stress.

(a) Retinal images of 7 dpf wild-type (upper rows) and clrn1^-/- mutant (lower rows) zebrafish expressing either rho:GFP or rho:Clrn1 transgenes (column labels) raised in control lighting conditions (right) or treated from 5-7 dpf with 3500 lux for 48 hours (left, light stress). Hoechst (blue) highlights nuclei of the outer (photoreceptor layer) and inner nuclear layers (labeled ONL and INL) while TUNEL staining (red) highlights dying cells. Quantitation of (b) pyknotic nuclei or (c) TUNEL-positive cells in the ONL reveals that re-expression of Clrn1 in rod photoreceptors does not protect clrn1^-/- mutants from light stress induced cell death. (*p<0.05, **p<0.01, ***p<0.005, ****p<0.001; Two-way ANOVA; Significance shown compared to wild-type rho:GFP without light stress). Error bars=SD. Scale Bar = 50 µm. n=5-10 larvae for each genotype per condition.