FIGURE 5

FIGURE 5

Lineage tracing of cranial neural crest cells (CNCCs) in sox10:Dendra2. (A,B) For time‐lapse imaging of the prospective frontonasal and anterior maxillary prominence, photoconversion of the prospective frontonasal prominence (A) and prospective anterior maxillary prominence (B) was performed in sox10:Dendra2 transgenic zebrafish at 15 ss. As photoconvertible fluorescent protein Dendra2 is photoactivated by UV light (405 nm) from green to red, specific Dendra2‐expressing cells were labeled irreversibly and traced. The time‐lapse imaging was performed by 30 ss (Movies [Link], [Link], [Link]). (C–C″) To investigate the cell lineage of the prospective frontonasal prominence after 30 ss, photoconversion of the frontonasal prominence at 24 hpf (30 ss) were performed (C). The lineage was subsequently examined at 48 (C′) and 72 hpf (C″). The frontonasal prominence was labeled at 24 hpf (C) and differentiated into the medial part of the ethmoid plate at 48 hpf (C′) and 72 hpf (C″). (D–D″) To investigate the cell lineage of the prospective anterior maxillary prominence after 30 ss, photoconversion of the anterior maxillary prominence at 24 hpf (30 ss) were performed (D). The lineage was subsequently examined at 48 hpf (D′) and 72 hpf (D″). The anterior maxillary prominence was labeled at 24 hpf (D) and differentiated into a lateral part of the ethmoid plate (trabeculae) at 48 hpf (D′) and 72 hpf (D″). e, eye; ch, ceratohyal; ep, ethmoid plate; gc, gill cartilage; m, Meckel's cartilage; PA1, first pharyngeal arch. Scale bars: 100 μm.