Fig 3

Fig 3 RiboFlip is a Flp- and Cre-inducible knockdown cassette.

(a) Schematics of the alb locus showing the position of the RiboFlip integration and the details of the cassette in the T3H48-HHR OFF orientation, Flp-ON orientation, and Cre-ON orientation; T3H48-HHR antisense orientation is in red and sense orientation in green; RiboFlip includes six unique primer sites (P1-6), three universal CRISPR sites (U1-3), a β-globin terminator (bGH) downstream of the TagBFP, and recombination sites (LOX/FRT/ROX). (b) Relative alb mRNA levels in 36 hpf alb^R-OFF/R-OFF, alb^{R-Flp-ON/R-Flp-ON}, and alb^{R-Cre-ON/R-Cre-ON} embryos; n=3 biologically independent samples; Ct values are listed in S1 Table. (c-f) Brightfield images of 36 hpf alb^R-OFF/R-OFF (c), alb^{R-Flp-ON/R-Flp-ON} (d,f), and alb^{R-Cre-ON/R-Cre-ON} (e) embryos non-injected (c-e) or injected at the one-cell stage with Cre mRNA (f). The proportion of embryos matching the image shown is indicated in the top right corner of each image. The diagram indicates the Anterior-Posterior (A-P) and Dorsal-Ventral (D-V) axes.