Fig. 5.

Fig. 5.

Upregulation of anti-hypercalcemic hormone stc1a in sox10 mutants. (A) Volcano plot of whole-body bulk RNAseq shows stc1a as significantly upregulated in the sox10 mutants at 4 dpf (P-value cutoff: 0.05; fold change cutoff: 1.25). Red arrow points to stc1a. (B,C) Both semi-quantitative rt-PCR (B) and in situ hybridization (C) detect a robust upregulation of stc1a mRNA in sox10 mutants at 4 dpf (unpaired t-test; P<0.0001, d.f.=4). Red boxes in C indicate the regions shown at higher magnification on the right. Numbers in panels indicate the proportion of larvae of that genotype with the presented phenotype. Scale bars: 100 µm. (D,D′) The increase in stc1a transcript levels is due at least in part to an increase in the number of stc1a⁺ cells in sox10 mutant corpuscles, first detected at 45 hpf and resolving at 7 dpf (unpaired t-tests; 36 hpf: P=0.640, d.f.=11; 45 hpf: P=0.00009, d.f.=8; 72 hpf: P=0.002, d.f.=6; 96 hpf: P=0.00003, d.f.=10; 168 hpf: P=0.0007, d.f.=10. ns, not significant; WT, wild type. Scale bars in D: 10 µm.