ZFIN Image: Cerveró-Varona et al., 2025, Figure 7

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Figure Caption

Figure 7

AEC_MV cargo transfer promoted an immunosuppressive response in PBMCs and Jurkat cells

(A) PHA-stimulated PBMCs ± Anti-CD44 and ± EIPA were treated with the different CM (AEC ± LPS_MV and AEC± LPS_MV-free) for 48h and assessed for their proliferation. Data were normalized on PHA-stimulated PBMCs (100% of proliferation).

(B) CD3/CD28-stimulated Jurkat reporter cells ± Anti-CD44 and ± EIPA were evaluated for the inhibition of NFAT activation after treatment with the different CM (±LPS) for 48h. Data were normalized on CD3/CD28 stimulated Jurkat (100% of NFAT activation). Data (mean ± SD) represent 3 independent sets of experiments (n = at least 3 biological replicates in each group per set; each biological replicate assayed in at least 3 technical replicates). ALL, ∗, ∗∗, ∗∗∗, and ∗∗∗∗ Statistically significant values between the different studied groups (p < 0.01, p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively). One-way ANOVA was employed for comparing normally distributed data.

Acknowledgments

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