Figure 6

Figure 6

Interspecific in vivo transfer of AEC-MV mitochondria cargo

(A) Illustration describing the experimental plan followed for the treatment of 72 hpf zebrafish larvae with AEC ± LPS_MV, and time points for the subsequent analyses.

(B) Representative confocal microscopy images of Tg (mpx:GFP) larvae after 6 h post damage (caudal fin cut) and treatment with AEC ± LPS_MV reportig the localization of ovine mitochondria MV (MitoTracker red), zebrafish neutrophils (green) and cell nuclei (blue) in the caudal fin.

(C) Quantification by qPCR of the amount of ovine mtDNA copy number contained in the zebrafish larvae after 24 and 48 hpd. (CTR: untreated zebrafish larvae; CTR+: AEC_MV per se). Data (mean ± SD) represent 3 independent sets of experiments (n = at least 10 biological replicates in each group per set). All, and ∗ Statistically significant values between the different studied groups (p < 0.0001, and p < 0.05, respectively). One-way ANOVA was employed for comparing normally distributed data. Scale bar, 100 μm.