Figure 5

Figure 5

Mitochondrial tracking demonstrated the iAEC-derived MV cargo internalization in immune cells

(A) Experimental design for B-C.

(B) Representative confocal images of co-immunofluorescence staining of nuclei (Hoescht 33342), cytoplasm (Calcein AM), and mitochondria-targeted turbo red florescence protein (TurboRFP-labeled mitochondria), in Jurkat ± EIPA exposed to iAEC_MV for 12h.

(C) Flow cytometric investigation to confirm the mitochondria MV internalization (TurboRFP-labeled mitochondria) in Jurkat ± EIPA. Data are presented as % of cells positive to TurboRFP. Data (mean ± SD) represent 3 independent sets of experiments (n = at least 3 biological replicates in each group per set; each biological replicate assayed in at least 3 technical replicates). ∗∗∗ Statistically significant values between the different studied groups (p < 0.001). One-way ANOVA was employed for comparing normally distributed data. Scale bar, 5 μm.