Figure 3

Figure 3

Protein content-based MV fraction characterization confirmed the presence of functional mitochondria

(A) Flow cytometric analysis of CNX, CD63, TOMM40, and HSP60, in AEC ± LPS_MV. Data were presented as mean fluorescence intensity (MFI) ratio over negative CTR.

(B) Representative WB images and relative densitometric analysis of HSP60 (normalized on total protein content via stain-free detection) in AEC ± LPS_MV.

(C) JC1 flow cytometric analysis to evaluate AEC ± LPS_MV ΔΨM. Data were presented as MFI ratio over negative CTR. Data (mean ± SD) represent 3 independent sets of experiments (n = at least 3 biological replicates in each group per set; each biological replicate assayed in at least 3 technical replicates). ∗∗ and ∗∗∗∗ Statistically significant values between the different studied groups (p < 0.01 and p < 0.0001). One-way ANOVA and two tailored t-tests were employed for comparing normally distributed data.