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Fig. 5 Loss of Smyd3 enhances the cellular antiviral immune response. (A) qPCR analysis of Ifn?, Isg15, and Ifit1 mRNA in Smyd3+/+ and Smyd3?/? BMDCs UI or infected with VSV for 8 h. (B) qPCR analysis of Ifn?, Cxcl10, and Ifn? mRNA in Smyd3+/+ and Smyd3?/? BMDCs UI or infected with HSV-1 for 8 h. (C) Microscopic imaging of VSV-GFP virus replication in Smyd3+/+ and Smyd3?/? BMDCs. (D) Microscopic imaging of HSV-1-GFP virus replication in Smyd3+/+ and Smyd3?/? BMDCs. (E) Phosphorylation and dimerization of Irf3 in Smyd3+/+ and Smyd3?/? BMDCs infected with VSV for 0, 4, and 8 h. (F) qPCR analysis of Ifn?, Cxcl10, and Ccl5 mRNA in Smyd3+/+ and Smyd3?/? MEF cells transfected with (+) or without (?) poly I:C for 8 h. (G) qPCR analysis of Ifn?, Cxcl10, and Ccl5 mRNA in Smyd3+/+ and Smyd3?/? MEF cells transfected with (+) or without (?) poly A:T for 8 h. (H) qPCR analysis of Ifn? and Cxcl10 mRNA in Smyd3+/+ and Smyd3?/? MEF cells UI or infected with VSV for 8 h. (I) qPCR analysis of Ifn? and Cxcl10 mRNA in Smyd3+/+ and Smyd3?/? MEF cells UI or infected with SeV for 8 h. (J) qPCR analysis of Ifn? and Cxcl10 mRNA in Smyd3+/+ and Smyd3?/? MEF cells UI or infected with HSV-1 for 8 h. (K) Microscopic imaging (Left panels) and immunoblotting (Right panels) of VSV-GFP virus replication in Smyd3+/+ and Smyd3?/? MEF cells. (L) Phosphorylation and dimerization of Irf3 in Smyd3+/+ and Smyd3?/? MEF cells transfected with poly I:C for 0, 4, and 8 h. ***P < 0.001 and ****P < 0.0001. Graphs represent fold induction relative to the untreated cells. Data are presented as the mean values of a representative experiment performed in triplicate (A, B, and F?J) or as representative data (C?E, K, and L); these experiments were repeated independently at least three times, and error bars indicate S.D.