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Fig. 4 - Supplemental 3 Mineralization in the skeleton at 90 dpf. Representative images of skeletal mineralization following Alizarin Red S (ARS) staining in a second clutch, demonstrating the consistency of the observed skeletal phenotype. The three genes on the left are associated with the pathogenesis of osteoporosis, while the last five genes on the right are linked to osteogenesis imperfecta. Images show specific crispants from a lateral view, captured using a Leica microscope. Scale bars = 1 mm.
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