Fig. 3 NAT10 promotes GC cell proliferation, migration and colony formation in vitro. (a) Determination of mRNA level after knocking out or over-expressing NAT10 by qRT-PCR assay. (b, c) Determination of protein level after knocking out or over-expressing NAT10 by western blot assay. (d) Growth curves of cells with NAT10 knockout or overexpression were determined using CCK-8 assays. (e, f) The wound healing of NAT10 knockout or overexpression (for 0, 24, 48 h) were recorded and quantitatively analyzed. (g–i) Colony formation assays were conducted to assess the proliferation of GC cells (left), and the results were quantitatively analyzed (right). GC, gastric cancer.
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