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Fig. 1 Image analysis results of immunohistochemical staining of human protein map. (a) The tissue segmentation and cell recognition outcomes between normal stomach tissue and stomach cancer. (b) There was a statistically significant difference between the average and integral optical densities of NAT10 cells in stomach cancer and normal stomach tissue. Different sections of stomach cancer and normal stomach tissue showed statistically significant values for the NAT10 cell number (c), positive rate (d), and density (e). (f) Comparison of NAT10 cell count and optical density readings across several stomach cancer locations. Student's t-test (meet the normal distribution) and Mann–Whitney U-tests (do not meet the normal distribution). *p < 0.05. ns, no significance.