ZFIN Image: Xia et al., 2025, Figure 9

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Figure Caption

Figure 9

Immunofluorescence results depicting NCC proliferation through antiphosphohistone H3 (PH3) staining in (A,E,I,M) control siblings, (B,F,J,N) ctdsp2^−/− mutants, (C,G,K,O) ctdsp2^−/− embryos injected with ctdsp2 mRNA, and (D,H,L,P) tp53-knockout ctdsp2^−/− embryos at 48 h post-fertilization (hpf). The merged images highlight the anti-PH3 signals in the NCCs located in the pharyngeal arch region, marked by a dotted box. (Q,R) Number of proliferating NCCs among each group. Note: Immunofluorescence experiments were performed in triplicate, statistical analyses were carried out using 3 randomly picked embryos in each group, and statistical significance was assessed by unpaired t-tests, with p < 0.05 considered statistically significant; **** p < 0.0001.

Acknowledgments

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