ZFIN Image: Xia et al., 2025, Figure 4

Image

Figure Caption

Figure 4

(A–C) Phenotypes, (D–F) Sanger sequencing chromatograms, and (G–I) genotypes of F2 embryos. The F2 generation displayed two distinct phenotypic categories and three different genotypes: embryos without deformities had either wildtype or heterozygous ((−3+1) bp indels) genotypes, while those with deformities were exclusively homozygous for the (−3+1) bp indels. The red box denotes the sequence of gRNA (exon 1) (GGT TGT CTT CGA AAA CAG GT AGG), and the blue box indicates the 3 bp deletion sequence (GGT) and the 1 bp insert (A).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.