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Fig. 5 Overall analysis of the transcriptome of zebrafish larvae inoculated with pcDNA3.1-igldcp or the empty control plasmid pcDNA3.1 in the absence or presence of RGNNV infection. A) Stacked column chart representing the number and intensity (in log2 of the fold-change) of the DEGs in different comparisons. (B) Principal component analysis (PCA) plot constructed with the TMP values of the entire repertoire of zebrafish genes. C) Venn diagram constructed with the DEGs obtained for the comparisons of pcDNA3.1-igldcp vs. pcDNA3.1 and pcDNA3.1 RGNNV vs. pcDNA3.1, revealing the common and exclusive genes affected by Igldcp overexpression and RGNNV infection. D) Venn diagram constructed with the DEGs obtained for the comparisons pcDNA3.1-igldcp vs. pcDNA3.1 and pcDNA3.1-igldcp RGNNV vs. pcDNA3.1 RGNNV, which allows the identification of DEGs modulated by Igldcp both in the absence and presence of infection and DEGs modulated by Igldcp only in the absence of infection or only in the presence of infection. E) Venn diagram constructed with the DEGs obtained for the comparisons pcDNA3.1-igldcp RGNNV vs. pcDNA3.1 and pcDNA3.1- RGNNV vs. pcDNA3.1, showing the number of common and exclusive DEGs modulated by infection in both groups of larvae (overexpressing or not overexpressing igldcp).