Fig. 1

Fig. 1 Mcm5 regulates T lymphocyte maturation during hematopoiesis.

A In control sibling embryos, the expression of rag1, rag2, il7r and ikaros was examined at 5 dpf (shown in the upper-row images). In mcm5^−/− embryos, the expression of rag1(n = 29), rag2 (n = 31), il7r (n = 19) and ikaros (n = 20) completely disappeared at 5 dpf (shown in the bottom-row images). BGH1 is a pituitary maker, here working as a control. Comparing to siblings (n = 30), the expression of GH1 in mcm5 mutants was normal (n = 27). C The cells labeled with DsRed (in Tg(rag2:DsRed)) disappeared in mcm5^−/− embryos at 5 dpf (n = 27). D Expression of rag2 was greatly decreased in mcm5^−/− embryos at 4 dpf (D, n = 27). The expression of GH1 was normal in mcm5^−/−embryos at 4 dpf (D, n = 21). E Quantification of the expression area of rag2 in siblings and mcm5^−/− embryos at 3.5 dpf and 4 dpf was showed. F The expression of rag1 in mcm5^−/− embryos was significantly downregulated at 4 dpf in the thymus (n = 30). Mcm5 mRNA injection restored the expression of rag1 in mcm5^−/− embryos (n = 28). G The quantification of the expression area of rag1 at 4 dpf is showed. For (E,G), the data were presented as means ± SD; The P-values (t-test; two-tailed); Ns not significant; *** P < 0.001.